National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Targeting Induced Local Lesions in Genome (TILLING) is a general reverse technique that combines chemical mutagenesis with PCR based screening to identity point mutations in regions of interest. The strategy works with a mismatch-specific endonuclease to detect induced or natural DNA polymorphisms in gene of interest. The main principles of TILLING include making EMS-mutagenized population, targeting gene of interest with PCR primers and searching rare mutants among many individuals. A study based on applying TILLING to elucidate gene function in a chemically induced sorghum mutant population showed that total of five mutants were detected for four gene targets. High throughput TILLING was demonstrated applicable to maize, with a large genome but with limited genetic resources currently available. This study also concluded public TILLING service for maize, modelled on the existing Arabidopsis TILLING Project. The basic applications of ECO-TILLING include mapping, association analysis, mutation profiling and biodiversity. The main advantage of TILLING is its ability in determining the spectrum of variation and for genetic mapping based on linkage association analysis. The technique is a however cost effective too. One of the basic disadvantages of TILLING is the low rate of induction of mutation and requirement of skilful labours.
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