National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Oil contaminating in the soil will lead to serious pollution problems. Bioremediation involves the use of organisms to remove pollutants from a contaminated site. Biosurfactant are structurally diverse compounds mainly produced by hydrocarbon utilizing microorganisms which makes them potential candidates for enhancing oil recovery. Pseudomonas aeruginosa PSPA15 strain is isolated from oil contaminated soil. It is screened to confirm the ability in the biosurfactant production. The surface tension value is 26mNm-1 and the emulsification indices are 83%, 79%, and 77% with petrol, diesel and kerosene respectively. The sequence of the 16srRNA and PSPA15 are compared and it shows 100% similarity to Pseudomonas aeruginosa gene bank. Biocompatibility assay exhibited no cytotoxicity against VERO cells. HPLC analysis shows retention time of 4.37 representing the rhamnolipids type of biosurfactant.
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