National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Increased and continued use of the diverse genotypes is a prerequisite for developing and diversifying the genetic base of crop cultivars. DNA markers which are crop-stage non-specific environmental neutral, easily assayable and amenable for automation are being used to assess the diversity of germplasm accessions and/or breeding lines. DNA markers also provide information on the population structure, allelic richness, and parameters that specify diversity among the genotypes to help breeders to choose those most appropriate for use in cultivar development. Hence SSR markers were used to assess diversity at marker loci among 16 phenotypically diverse dolichos bean genotypes. In the present study, 52 of 55 SSR-based markers were polymorphic, resulting in 94.55% polymorphism. Amplification of genomic DNA segments complementary to 55 SSR primers resulted in 133 scorable alleles with an average of 2.5 alleles per SSR loci. SSR markers exhibited differential ability to discriminate 16 genotypes as indicated by the estimates of effective multiplex ratio which ranged from 1.89 to 4.73 and marker index ranged from 0.69 to 3.40. The average gene diversity in the present study is more than that reported in dolichos bean. The estimates of Shanon’s diversity index complemented those of average gene diversity. These results indicate that these SSR markers are highly informative and could be used to assess genetic diversity among the genotypes. The genotypes, HA 10-8, FPB 15 and RIL 162 share different alleles, FPB 8 and RIL 21 share similar alleles. Hence, the genotypes, HA 10-8, FPB 15 and RIL 162 could be used in crossing programme to derive genotypes with combination of desired traits.
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