Strawberry (Fragaria x ananassa Duchesne) is one of the most important commercial plants representing Rosaceae family. Like other fruit crops, strawberry is also known to be susceptible to diseases caused by fungi, bacteria and viruses. Since strawberry is propagated through runners year after year, the mother stocks often get infected with viruses pass on these viruses to the next progenies. Reports indicate that more than 30 viruses and phytoplasmas infect strawberry naturally. The most important viruses are strawberry latent ringspot virus (SLRSV), tobacco ringspot virus (TRSV), raspberry ringspot virus (RRSV), tobacco streak virus (TSV) and strawberry Mild yellow edge virus (SMYEV). A detailed investigation was conducted to characterize the major viruses infecting strawberry on serological basis using both Direct Antigen Coating (DAC) and Double Antibody Sandwich (DAS) forms of ELISA. The studies found DAS-ELISA to be more precise than DAC-ELISA for serological characterization of strawberry viruses.

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