National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Region wise monitoring of the PPR virus by active surveillance is essential as it aids in strategic planning of measures of disease control and prevention. The North Eastern Karnataka with sizable sheep and goat population experiences the outbreaks of PPR but the attempts to isolate and characterize the prevailing PPR virus is limited. Hence, the present research work was carried out with the objective of isolation and characterization of PPR virus isolates circulating in North Eastern Karnataka by microscopic (TEM) and molecular (RT-PCR) methods. A total of 15 suspected PPR outbreaks were attended and different types of clinical samples like nasal, oral, ocular, rectal swabs and blood from ailing kids and goats were collected. The samples were initially screened for the presence of PPR virus by observing the infected Vero-cells for development of characteristic CPE. The isolates showing CPE were further confirmed for the virus by RT-PCR using N and F genes of PPR virus. The PCR confirmed isolates were subjected to transmission electron microscopy (TEM). A total of six PPR isolates were obtained representing five districts of North Eastern Karnataka which were confirmed by RT-PCR. The TEM images showed pleomorphic virion particles with varying sizes indicative of Morbilli-virus. These findings provide the first documentation of PPR presence by microscopic and molecular characterization in the region.
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