National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Downy mildew, caused by Sclerospora graminicola (Sacc.) Schroet, is a disease of great economic signiï¬Âcance in the production of pearl millet (Pennisetum glaucum). Host physiology particularly host metabolites and oxidising enzymes get disturbed due to attack of Sclerospora graminicola. Altered state of oxidative enzyme plays an important role in plant metabolism during pathogenesis. In the present investigation biochemical characterization of pearl millet genotypes - HHB 223 and 7042 S was carried out at 30, 40, 50 and 70 DAS. Total phenols were higher in resistant genotype at 30 DAS in comparison to susceptible genotype but with the progression of disease total phenols decreased in resistant genotype at 40, 50 and 70 DAS but in susceptible genotype they increased. Orthodihydroxy phenol was higher in resistant genotype at all the stages in comparison to susceptible genotype. Flavanols were higher in resistant genotypes at 30 DAS in comparison to susceptible genotype. With the progression of the disease, flavanol content decreased in both the genotypes but more reduction was observed from 30 DAS to 70 DAS in susceptible genotype. Total chlorophyll and carotenoid content decreased with the progression of systemic infection in both resistant and susceptible genotypes. Total soluble sugars were higher in resistant genotype in comparison to susceptible genotype. Peroxidase and catalase activities were higher in susceptible genotype at early stages of growth but at later stages higher in resistant genotype. Polyphenol oxidase did not show any significant difference in resistant and susceptible genotype. Phenylalanine Ammonia Lyase activity was higher and continuously increased in susceptible genotype at all stages of growth whereas Tyrosine Ammonia Lyase activity was found in traces in both the genotypes. Protein content was initially higher in susceptible genotype at 30 DAS and 40 DAS but it decreased with disease progression. Change in the activities of antioxidant enzymes and in various metabolites can be used as a biochemical marker for the degree of resistance.
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