National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Biofilm is the population of bacterial cells growing on the biotic and abiotic surfaces embedded in a matrix of extracellular polysaccharide, which facilitate the adherence of microorganism. This study is aimed to compare and evaluate different Methods for detection of Biofilm formation in Staphylococcus aureus isolates. For comparison of different methods of Biofilm production, 100 isolates of Staphylococcus aureus (50 MRSA and 50 MSSA) were taken and screened by tissue culture plate method, tube method in different condition and Congo Red Agar (CRA) plate method. Out of 100 isolates of Staphylococcus aureus, 19% shows Strong biofilm formation, while 33 % showed moderate and 48 % isolates showed weak/No biofilm production by TCP Methods. In TM-Gr-1: 10%, 28% and 62%; in TM-Gr-2: 10%, 20% and 70%; in TM-Gr-3: 16, 29% and 55% isolates showed strong, moderate and weak/ No biofilm production respectively. The CRA Method showed 17% biofilm production. The TCP method was found to be most sensitive screening method for detection of biofilm formation by staphylococcus aureu. MRSA isolates were more Biofilm producers than MSSA.
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