National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salmonella is a major foodborne bacterial pathogen responsible for millions of cases of infection and thousands of death in humans across the globe annually. Poultry and poultry products are considered to be the major source of Salmonella infection in humans. The economic loss caused by salmonellosis is very huge in case of poultry industry. Vaccination of the poultry is found to efficiently reduce the zoonotic risks associated with Salmonella infection. Virulence nature of outer membrane proteins of Gram-negative bacteria Salmonella is found to efficiently generate immunity in host system. Hence, several efforts have been made to develop an outer membrane protein based vaccine and were found to be successful. So, in the present study outer membrane protein F (OmpF) of Salmonella typhimurium was cloned, sequenced and in silico analysis was carried out to study its structural and immunological characteristics. Antigenic indexing, Epitope mapping and MHC mapping were performed using various prediction servers and online tools. Presence of eight major B cell epitopes indicated ability of OmpF to elicit humoral immunity. VaxiJen score of 0.85 further confirmed the antigenic nature of the epitope. T-cell epitope mapping identified several epitopes with high MHC binding affinity representing ability to provoke cell mediated immunity. The results from the study indicated that OmpF protein of Salmonella typhimurium is a strong candidate for developing recombinant vaccine against salmonellosis in poultry.
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