National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Citrus tristeza disease (CTD) is the most dangerous and lethal viral disease among all the viral diseases that attack to the citrus. It is caused by Citrus tristeza virus (CTV) belongs to the genus Closterovirus in the family Closteroviridae. For quick and reliable detection of CTV a one-step RT-PCR technique was standardised. The total RNA from the leaf sample was extracted by using the Quiazen RNAeasy plant mini kit. The RT-PCR kit was used for the RT-PCR reaction and the primers specific to the coat protein component of the virus is used for detection. The PCR products were subjected to the gel electrophoresis on the 1% agarose gel containing 2.5 ug of ethidium bromide (Edbr). After gel running these products are visualized under UV transilluminator. Where the CTV positive samples yield an amplicon of 672 bps but where as in negative samples there was no such amplicon. Therefore the standardized RT-PCR is found to be most sensitive and reliable method for quick detection of CTV in the infected samples.
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