National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacteriocins and Bacteriocin like Inhibitory Substance (BLIS) are ribosomal products exhibiting antimicrobial activity. The present study aimed to screen for production of antimicrobial agents from soil bacteria of dairy environment. Borer was used to dig soil and collected in sterile plastic bags. Isolation was done on tyrosine agar. To exclude non spore formers the soil suspension, was heated at 80ºC. The bacterial isolates were identified underpinning biochemical and carbohydrate utilization tests and screened for their antibacterial activity against methicillin resistant S. aureus and E. coli by agar well diffusion method. Among forty nine isolates three isolates were identified as Brevibacillus brevis. Screening of crude brevicin obtained from cell free broth inoculated with B. brevis exhibited a strong antagonistic activity against S. aureus but no zone of inhibition was recorded against E. coli. The spore forming bacterium, B. brevis produced narrow spectrum antibacterial agent. Strains of Brevibacillus are potent source of bacteriocins and these antimicrobial peptides can be used as an effective therapeutic agent in pharmaceuticals as well as can also be used as food preservatives in canned or packed food and dairy products, controlling pathogens with natural products instead of synthetic ones.
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