National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Minimum Inhibitory Concentration (MIC) of 29 Lactobacillus isolates was determined by E-test strip method according to CLSI and EFSA, 2012 standards. All isolates displayed resistance towards aminoglycosides while 63% of isolates were found resistant to amikacin (MIC 16-256 µg/ml). Strains S8a and S8b (L. rhamnosus and L. acidophilus) were resistant to tetracycline (MIC 256 µg/ml). Isolates displayed resistance to quinolones [nalidixic acid with MIC 256 µg/ml; norfloxacin, ofloxacin, ciprofloxacin and levofloxacin with MIC 6-256 µg/ml; and sparofloxacin (MIC 0.25-4 µg/ml)], azolidiones [nitrofurantoin (MIC 3-256 µg/ml)] and cephalosporins (MIC 256µg/ml). Seventy-three percent of isolates displayed resistance against vancomycin while 66% to teicoplanin. Isolates, S1b (L. reutri), S4 (L. reuteri); S5 (L. plantarum); S8a (L. rhamnosus) and S8b (L. acidophilus) exhibited resistance towards beta-lactams. Very few strains exhibited susceptibility to class macrolides [roxithromycin (S10b- L. plantarum; MIC 0.5 µg/ml); clarithromycin (S6a- L. plantarum MIC 0.125 µg/ml); (S12- L. acidophilus MIC 0.25 µg/ml) and azithromycin (S12- L. acidophilus MIC 0.75 µg/ml)]. The present study found the prevalence of antibiotic resistance among commercial available probiotics, which may pose a safety risk among humans. Hence, antibiotic sensitivity should be considered as essential for the evaluation of safety assessment of probiotics.
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