National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Study was conducted to isolate the lipase producing bacteria and characterize the lipase enzyme for its optimal activity. The samples were collected from vegetable oil spillage site. A total of 26 isolates producing lipase were isolated from three different samples processed. Lipolytic activity was confirmed by observing the zone of hydrolysis formed around the growth of colony on TBA plate. Out of 26 isolates, 4 isolates, viz A2, B4, C1 and C2 having zone diameter more than 1 mm were considered to be potential isolates and were subjected for further characterization. Lipase was quantified by determining the EU/ml by butyric acid titrimetric assay. All the potential isolates were also subjected to morphological and biochemical characterization for their partial identification. B4 and C1 isolates from 4 selected isolates were Gram positive while remaining A2 and C2 were Gram negative. Isolate B4 and C1 were identified as belongs to genus Bacillus, while other isolates required further characterization for their identification. Lipase enzyme produced by these potential isolates was also characterized for determining its optimal activity. Effect of different pH and temperature on lipase activity was determined. The optimum pH for A2, B4, C1 and C2 was found to be 7.5, 8, 8 and 7.5 respectively, while the optimum temperature for lipase activity for A2, B4, C1 & C2 was found to be 37ºC respectively
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