National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Sepsis is a major cause of morbidity and mortality among neonates. Neonatal sepsis may be categorized as early-onset or late-onset. Clinical manifestations of neonatal sepsis are non-specific, therefore, clinical diagnosis of sepsis is difficult and laboratory help is required. Present study was done to evaluate three different methods in diagnosis of neonatal sepsis. 3 ml of venous blood was collected employing strict aseptic precautions from all babies admitted to the NICU in our hospital during the study period. 2 ml of blood was aseptically added to BacT/ ALERT PF culture bottle. Bacteria grown from these bottles were identified by using the standard protocol. The antibiotic susceptibility for the isolates was tested by Kirby Bauer method following CLSI guidelines. Remaining blood sample was used for PCT estimation by immunochromatography assay and CRP estimation by chemiluminescence method. 50 blood samples were collected from neonates admitted to the NICU. The most common risk factor of suspected septicaemia in our study was birth asphyxia (32%), followed by preterm birth (28%). Blood culture was positive in 12 (24%) cases. The most common etiological agent was Staphylococcus aureus. PCT was positive in all blood culture positive (proven sepsis) cases. Out of 50 samples tested for CRP, 33(66%) were positive. A wide range of CRP values were obtained, ranging from 1.83 mg/dL to 120 mg/dL. Out of the 34 PCT positive cases, 33 (97.05%) were also CRP positive. Blood culture even though considered as gold standard it is time consuming and at times gives false negative results due to administration of antibiotics. PCT and CRP are reliable markers which aid in diagnosis of neonatal sepsis, which have the same diagnostic accuracy. CRP, when compared to PCT is affordable and can be conveniently used as a marker for the diagnosis of neonatal sepsis, especially in developing countries with poor resources
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