National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Banana (Musa spp.) is important fruit crop in India. Banana is one of the oldest and most popular fruit grown in India with great socio-economic significance. The present study aims to assessment of clonal fidelity and genetic analysis of tissue culture raised banana (Musa spp.) plant. Banana tissue culture propagation is a new an established a popular technology due to the advantages. Through plant tissue culture method large scale production is possible in short time. The most reliable methods are the molecular marker techniques that identify the variance depending on the plant proteins, which are expressed from defined regions of DNA, or DNA polymorphisms. RAPD (random amplified polymorphic DNA’s) is a powerful technique for identification of genetic variation (Welsh et al., 1990). The RAPD technique is effective to develop genotype-specific banding patterns valuable for cultivar identification. Molecular analysis using RAPD’s can be applied to assess the clonal fidelity of plants derived in-vitro on an industrial scale as part of crop improvement programs. With the primer OPA-19 and OPC-1 amplified 1 mother and 4 daughter subculture are showed polymorphic bands is seen range of 500bp to 1000pb.
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