National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The cultivated tomato (Solanum lycopersicum L.) is economically one of the most important and widely grown plants of the Solanaceae family. Epidemics of Tomato leaf curl virus associated with upsurge of whiteflies (Bemasia tabacci) on tomato crop has been frequently reported which up to 100% yield losses (Shelat, et.al, 2014). In all twenty six samples were collected to see the presence of tomato leaf curl virus (ToLCV). Polymerase chain reaction (PCR) in the moist sensitive approach to detect a minute amount of viral nucleic acid it is the most method to amplified geminvirus as they replicate via-double-stranded, circular DNA from. In this study, geminivirus specific detergents primer was employed to detect ToLCV occurring in it vector whitefly Bemisia tabaci by PCR based approach. The presence of virus was observed through PCR method with specific primer CPAV1 forward and reveres on different annealing temperature range from 52ºC to 56ºC. The presence of virus was observed at 54ºC annealing temperature in sample no. 4, 11, 15, 22, 24, 25, 26) and confirm the presence of ToLCV.
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