National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was taken up to know the effect of corpus luteum (CL) on the oocytes retrieval, quality in buffalo ovaries. Ovaries were collected from apparent healthy slaughtered buffalo during breeding season and categorized based on the presence (or) absence of CL. The cumulus oocyte complexes (COCs) were aspirated aseptically from the follicles of >6 mm diameter present on the surface of the ovary by using 18 G needle attached to 5 ml disposable syringe containing 2 ml of collection media. The oocytes were graded based on the presence of the cumulus cells complex around the oocytes. The mean A grade (3-5 cumulus cell layer) oocyte recovery rate per ovary was 1.35 from the ovaries with CL, while those without CL were 1.97, and the mean oocyte recovery rate per ovary was 1.78. In conclusion, an ovary without CL was found to be more suitable for harvesting higher number and superior quality of COCs.
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