National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Experiment was conducted during 2016-2017 on twenty cultivars/genotypes of rapeseed and mustard to understand the molecular variability using RAPD and its reaction against Albugo candida. Artificial inoculation was done by sporangial suspension and the overall average disease incidence ranged from 4.16-23.42 per cent among the tested genotypes/cultivars. RAPD analysis on twenty cultivars/genotypes of rapeseed and mustard with OPB-01, OPB-08 and OPD-13 primers revealed that OPB-01 had the highest polymorphic information content. At a distance coefficient of 0.25, twenty cultivars/genotypes were cluster into 2 major clusters, the first major cluster consists of 19 cultivars/genotypes and second major cluster consist of only 1 cultivar/genotype. Using 3 RAPD primers, all the 20 cultivars/genotypes were successfully classified except 4 cultivars/genotypes.
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