National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bio-nanotechnology has provides extensive research with emergence in biotechnology and nanotechnology which synthesize nanoparticles using bio-organisms like plant parts, bacteria yeasts, algae and fungi and that is more compatible with the green chemistry principles. Copper nanoparticles were biologically synthesized by using the root extract and leaf extract of Asparagus adscendens Roxb. as a reducing agent. By treatment of aqueous solutions of CuSO4•5H2O with root and leaf extract stable copper nanoparticles were formed. UV-Visible study revealed quantitative formation of copper nanoparticles or characteristic absorption peak of Cu nano-particles in both cases at the range of 500-700nm. These biosynthesized Cu nanoparticles were characterized with the help of Fourier transform infrared spectroscopy (FTIR) and High Resolution Transmission Electron Microscopy (HRTEM). The involvement of proteins and possible reducing agent was confirmed by FTIR analysis. HRTEM confirms the formation and the crystalline nature of copper nanomaterial. Thus, this method can be used as a rapid and environmental friendly, non-toxic and safe biological reagents approach for copper nanoparticles synthesis. The copper nanoparticles were isolated from these herbal roots and leaves tested for antimicrobial activity. The test cultures included in this study were Escherichia coli, Bacillus subtilis, Salmonella typhi, Klebsiella pneumonia, and Staphylococcus aureus. This study scientifically revealed significant zone of inhibition against such pathogenic bacteria’s which has many pharmaceutical applications for the control of deadly pathogens.
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