National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Trichoderma harzianum is a biocontrol agent that has moderate effect on soil balance and no harmful effect on other beneficial organisms in the soil. In this study we examined the effect of pH and temperature on the mycelia growth and spore yield of T. harzianum in batch and fed-batch culture. The pH and temperature had significant effect on the growth and sporulation of T. harzianum and this was dependent on the cultivation technique. It was observed that the optimum pH for maximum mycelial growth and spore yield produced by T. harzianum in batch and fed-batch cultures was pH 4 while the optimum temperature for mycelial growth in batch and fed-batch culture was 30 oC and the maximum spore yield was produced at optimum temperature of 25 oC and 45 oC in batch and fed- batch culture respectively. The results showed that fed-culture produced more spore yield than batch culture while high mycelial growth was obtained in batch culture. This work has revealed the important role that environmental conditions plays in the mycelia growth and spore yield of T. harzianum, a biocontrol agent.
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