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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:4, April, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(4): 488-494
DOI: https://doi.org/10.20546/ijcmas.2018.704.057


Studies of Histology, Histochemistry and Micrometry of Hippocampus in Surti Buffalo (Bubalus bubalis)
Harishbhai P. Gori, Subhash C. Dubal, V.A. Patel and K.M. Panchal
Department of Veterinary Anatomy and Histology, College of Veterinary Science and Animal Husbandry, Anand Agriculture University, Anand-388001, Gujarat, India
*Corresponding author
Abstract:

The brain of six adult surti buffaloes was collected for the study of hippocampus. Harris’ Haematoxylin and Eosin (H & E) Staining method was used for general microscopic structure of the hippocampus. Aldehyde-Thionin-PAS method was utilized for Nissl’s substance and mucopolysaccharides. Vogt’s method was used for nerve cells, Sevier-Munger method for neural tissues and the micrometry of hippocampus was performed by various layers of cornu ammonis and dentate gyrus. The mean value of thickness of different layers of cornu ammonis was measure by micrometry. Microscopic study revealed thatthe hippocampus was consist of six layers, viz.; alveus, stratum oriens, stratum pyramidale, stratum radiatum, stratum lacunosum and stratum moleculare. The hippocampus commissure and the dentate gyrus showed PAS positive reaction. Furthermore, these structures also showed a positive reaction with silver nitrate staining (Sevier-Munger staining). The present study revealed that almost all the fibres were myelinated fibers.


Keywords: Histology, Histochemistry, Micrometry, Hippocampus, Dentate gyrus and Buffalo

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How to cite this article:

Harishbhai P. Gori, Subhash C. Dubal, V.A. Patel and Panchal, K.M. 2018. Studies of Histology, Histochemistry and Micrometry of Hippocampus in Surti Buffalo (Bubalus bubalis).Int.J.Curr.Microbiol.App.Sci. 7(4): 488-494. doi: https://doi.org/10.20546/ijcmas.2018.704.057
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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