National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Nosocomial Klebsiella pneumoniae infections are particularly a problem among cancer and immunocompromised patients worldwide. K. pneumoniae strains are widespread in nature thus typing is required to discriminate them in the epidemiological investigations. In this study, the diversity of 43 nosocomial multi-drug resistant (MDR) K. pneumoniae isolates recovered from different clinical specimens collected from cancer patients at National Cancer Institute, Cairo, Egypt, were phenotypically and genotypically analysed. These isolates were identified using conventional microbiological methods and the API 20E system. Investigation of the antimicrobial susceptibility patterns against 16 diverse antimicrobial agents revealed that all isolates are MDR. The phenotyping was performed using the API 20E-based biotyping and antibiogram typing which showed three different biotypes and 25 antibiogram types among the isolates. The genotyping using random amplified polymorphic DNA (RAPD) analyses revealed 39 different RAPD-based fingerprints and/or 43 different patterns among the isolates. In conclusion, K. pneumoniae infections in this institution have been caused by diverse MDR K. pneumoniae genotypes and/or phenotypes clone groups with isolates in the same phenotype group possess different genotypes. Biotyping and antibiogram typing of K. pneumoniae isolates have been shown to be well for preliminary screening of strain relatedness. The use of RAPD-PCR-based analyses is recommended, which has high discriminatory power providing definite information to evaluate the epidemic status of the nosocomial infections caused by MDR K. pneumoniae.
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