National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Microsatellites or simple sequence repeats (SSR) are widely used in population genetic analysis because of their higher polymorphism, abundance, and co-dominance. The present work was taken to develop a specific primer for novel microsatellite loci and to validate them. Total 25 primer pairs were designed of which six primer pairs were found to produce specific band of the desired size. These six primers were validated using samples collected from Andhra Pradesh (Godavari River), Assam (Brahmaputra River), and West Bengal (Hooghly River). Out of six, five loci were found polymorphic with a 2-4 number of alleles per locus. Among the five loci, Cb21GACA locus showed maximum alleles (3-4) with highest PIC value (0.664) while locus CbACT6-GT25 showed less PIC value (0.164). The highest effective number of alleles was 2.721 in Cb21GACA and the lowest effective number was 1.241 of CbACT6-GT25. The mean effective number of alleles for overall populations was 1.847. The observed and expected heterozygosis was ranged from 0.05 to 0.80 and 0.095 to 0.66, respectively. The FST (0.164) and a number of migrants values (0.718) indicate a moderate level of population sub-structuring.
Citations |
 |
 |
 |
 |
