National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A Gram positive, motile, mesophilic Bacillus subtilis strain capable of producing extracellular protease was isolated from abattoir waste material which was collected from Wadala local market, Mumbai, India. In this work, the efficiency of mustard oil cake as a substrate for protease production by Bacillus subtilis strain in submerged fermentation (SmF) has been studied. The mustard oil cake was selected because it is rich in protein, cheap, abundantly available being an agro-industrial waste and can be constructively used as one of the main constituents in medium formulation. Different physical and chemical parameters such as pH, temperature, substrate concentration and incubation time were optimized. The maximum protease activity was found at medium pH of 11 containing 4% mustard oil cake at 40ºC, after 70 hours of submerged fermentation.Further, the growth curve studies indicated that the production of extracellular protease was initiated in the early lag phase and was maximum in the late exponential phase.
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