National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Genetic diversity among 20 cultivars of pigeonpea (Cajanus cajan (L) Millsp.) analysed using twelve SSR markers. Although most of the cultivars indicated polymorphism for the studied primers, cultivars BRG-2, BRG 5, GRG 818, HY3C, ICP 8863, ICPL 87119 and JKM 189 could amplify specific unique amplicon at higher molecular weight (138-148 bp) when primer CCB-8 was used. Similarly cultivars AKT 08-02, CoRG 9701, GRG 818, JKM 189, JKM 206, TS 3R, TTB, WRG 173 and WRP 1 could amplify specific unique amplicon at higher molecular weight (155-180 bp) when CCB-9 primer was used. In case of primer PB-3, cultivars AKT 08-02, BRG 1, BRG 3, CoRG 9701, GRG 822, ICP 8863 could amplify specific unique amplicon at higher molecular weight (280-340 bp) and primer PB-8 could amplify cultivars BDN 2, BRG 1, BRG-2, BRG 3, BRG 4, JKM 189, JKM 209, TS 3R, TTB-7 at higher molecular weight (305-320bp). Among the twelve primers (CCB-10, CCB-3, CCB-6, CCB-7, CCB-8, CCB-9, PB-1, PB-3, PB-7, PB-8, PB-9, PB-10) used in the present study for genetic diversity assessment CCB-8, CCB-9, PB-3 and PB-8 can be useful in detecting the genetic structure of the plants rapidly and easily by PCR technique via marker aided selection in early stages of growth.
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