National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was conducted to evaluate the combined effect of herbs on pre-freeze and post-thaw sperm motion traits in subfertile buffalo bulls. Three subfertile buffalo bulls which were maintained on the basal ration along with this, they were orally supplemented with Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves; Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight of bulls for 60 days of treatment phase. A total of 144 semen ejaculates (16 ejaculates/ bull/ treatment phase) were collected during pre-treatment, treatment and post-treatment phase (each phase of 60 days). Semen was extended with Tris egg yolk extender and freezing was carried out in a bio-freezer. Semen was evaluated for sperm motion traits in pre-freeze and post-thaw semen by Computer Assisted Sperm Analyser (CASA). Herbal supplementation significantly (P<0.05) increased the pre-freeze curvilinear velocity (VCL) during treatment phase and post-treatment phase, whereas VSL, STR and BCF were significantly (P<0.05) increased only during post-treatment phase. Moreover, herbal treatment significantly (P<0.05) improved the post-thaw individual motility during the treatment and post-treatment phase, whereas rapid progressive motility was significantly higher only during the treatment phase. It could be concluded that dietary supplementation of herbs in combination improved the sperm motion traits in subfertile buffalo bulls.
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