National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Type 1diabetes (T1D) is an organ-speciï¬c autoimmunity characterized by the invasion of auto-reactive T lymphocytes to the cells of the Langerhans islets that secreted insulin and the production of autoantibodies versus pancreas antigens. In order to underline the role of the genes involved in this study‚ we investigate ‚using PCR-RFLP for CTLA-4gene‚ PCR-RFLP and sequencing for VDR gene. Polymorphisms of two single nucleotide polymorphisms (SNPs) belonging to both genes in 60 T1D patients and 30 healthy control from Iraqi population. The present local study demonstrated that VDR gene FoK-I‚ FF genotype (P = 0.003) ‚Ff genotype (P = 0.002) and F‚ f alleles (P = 0.002) frequencies were significantly associated; VDR gene Bsm-IBB genotype (P=1.9×10-4)‚ bb genotype (P= 8.1×10-4) and B‚ b alleles (P=4.6×10-6) was significantly associated .CTLA-4-1722(T>C) was non significant association while +49 (A>G) GG genotype (P=0.036) and AA genotype (P=0.004) shows significant association. These result suggest the involvement of VDR and CTLA-4gene in the genetic susceptibility to T1D. Interestingly FoK-I‚ Bsm-I and +49 (A>G) contributes to increasing the risk to the disease in our population. However‚ Further studies are require to confirmed this finding especially the VDR gene andCTLA-4-1722(T>C) gene investigated for the first time in Iraqi population.
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