National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cold active lipase was investigated by bacteria and yeast isolates from the core sample of Nella Lake, Larsemann Hills region, East Antarctica. Among potential yeasts and bacteria producing lipases, best isolates were identified as Cryptococcus sp. Y-32 and Rhodococcus erythropolis N149 by molecular technique. In order to enhance the lipase production capabilities, both the isolate again subjected for optimization processes using various physiological (temperature and pH) and chemical (Carbon, nitrogen, minerals and various substrates like oils and triglycerides) parameters. The results indicated that a supplement of 1% w/v fructose, 0.1% w/v KCl and 2% v/v tween 80 at pH 8.5 and 15°C enhances the lipase production by 9.81-fold (6.81 U/ml) using Rhodococcus erythropolis N149. In other hands, the activators are 1% w/v of galactose and peptone, 0.1% w/v KCl and 2.5% v/v ghee at pH 11.5 and 15°C enhances the lipase production by 4.01-fold (3.35 U/ml) using Cryptococcus sp. Y-32. The present study successfully produced cold-active lipases with novel properties like low temperature and high pH stability, which can be used in the degradation of lipid wastes in cold regions and also can be used for detergent formulation for cold temperature washing of delicate clothes.
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