National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Begamoviruses are usually considered not transmissible by mechanical sap inoculation and hence making information about their physical properties scarce which are useful in determining management strategies. The present study was conducted to know sap transmissibility and physical properties of Mungbean Yellow Mosaic Virus (MYMV) like Thermal Inactivation Point (TIP), Dilution End Point (DEP) and Longevity in vitro (LIV). The infective sap prepared in potassium phosphate and sodium phosphate buffers of pH 7.5, 7.6, 7.7, 7.8, 7.9 and 8.0 at 0.1M inoculated on French bean and mungbean revealed that 0.1M potassium phosphate buffer at 7.8 pH could successfully transmit MYMV on French bean under glass house conditions. The determination of physical properties using the same buffer showed Thermal Inactivation Point (TIP) of MYMV as 50 0C for 10 minutes, Dilution End Point (DEP) was 10-4 and Longevity in vitro (LIV) was 1-2 days at 4 0C storage in vitro. The results add to the knowledge of MYMV transmission ability and its physical properties which helps in determining management strategies against the virus.
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