National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The omental adipose tissue was collected from, the animals undergone ovariohysterectomy surgical procedures. The samples were digested with 0.1 per cent (W/V) collagenase type I and transferred to a beaker with magnetic stirrer at 600 rpm at 37oC for 30 minutes. The lesser time was given for enzymatic digestion as compared to other species. The viability of the cell was evaluated by trypan blue exclusion method using haematocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the membrane in a dead cell and dye it blue. The isolated cells were seeded at a density of 1x106 cells per T25 culture flask and incubated in 5 ml of DMEM-HG at 37oC with 5 per cent CO2. 70-80 per cent confluency was observed on day six and 100 percent confluency on day seven.
Citations |
 |
 |
 |
 |
