National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Toxoplasma gondi being microscopic size and obligate intracellular, its proliferative forms cultivation in the laboratory is relative difficult and thus, immunodiagnostic tools such as serology and immune-histochemistry development becomes essential to demonstrate the infection in vivo. Among the various existing serological methods, the enzyme-linked immunosorbent assay (ELISA) is simple, rapid and economical, rendering it suitable for clinical diagnosis applications. The aim of present study was to express the microneme 10 (MIC10) antigenic protein which is a circulating antigen following invasion by tachyzoites in hosts in the prokaryotic expression system and evaluated as a potential diagnostic marker by indirect ELISA using the infected and non infected sera samples from Swiss albino mice. Optimum working dilutions of the antigen, sera and the conjugate were determined by checker board titrations and the cut off value was generated as mean plus standard deviation of optical densities of negative sera by ELISA. Recombinant MIC10-ELISA at the cut off value of 1.2539 showed the sensitivity and specificity as 79.2% and 87.2% respectively. The data was generated as a step to evaluate the potential of MIC10 antigen protein as a diagnostic marker.
Citations |
 |
 |
 |
 |
