National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was conducted to purify and characterize the active compound produced from Streptomyces parvulus for evaluating its cytotoxic potential against tumor cell line. The Streptomyces parvulus CBJ1, isolated from mangrove ecosystem of Poovar found to be an efficient producer of bioactive compound. The isolate produced yellow color in ISP2 production media during submerged fermentation. The orange color compound was obtained from mycelia extract using thin layer chromatography and purity was checked using reverse phase HPLC. The purified compound was identified as actinomycin D on the basis ofultraviolet-visible spectroscopy, mass spectroscopy and by comparison with published reference compounds. Theantitumor activity of purified compound, actinomycin D analyzed on A549 cell line by MTTassay. The actinomycin D showed cytotoxicity on A549cell line depending on the dose and incubation time. It showed 77% cytotoxicity at concentration of compound 1.25µg/ml with IC50 value of 0.52µg/ml.
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