National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Fusarium species cause considerable crop losses worldwide and necessitate an expensive use of chemical pesticides. Resistant genes can be used to improve plant resistance through gene editing and gene silencing strategies. Currently, information on host defences against Fusarium species is limited. Through in-silico tools, identification of genes and understanding of disease resistance mechanism has become possible. In the present study, a comprehensive in-silico analysis of 65 Fusarium resistance genes belonging to 10 different species was carried out to understand the physiochemical properties, secondary and tertiary protein structures, protein binding sites, phylogenetic relationship, cis-regulatory elements and protein motifs. Five genes on the basis of their stability have been selected for 3D structure prediction using Phyre2 server. These structures were evaluated and validated using Verify-3D, PROCHECK and Ramachandran plot. The Z score predicted by ProSA-web tool indicated reliability of the 3D structures while above 97% of the region falls in allowed region of Ramachandran plot. The distribution pattern in five genes for binding site is high, while hydrophobicity is very low to low. The most commonly occurring motifs among Fusarium resistance genes were motif-3 and motif-1, whereas cis-regulatory elements were TC-rich repeats, Box-W1, EIRE, AT-rich sequence, CGTCA-motif and TGACG-motif. The results of cis-acting regulatory elements and motif analysis revealed that they are actively involved in the expression and regulation of Fusarium resistance genes during cellular development and under biotic stress conditions in different plant species. In-silico analysis revealed the homology of Fusarium resistance genes and will be helpful to develop molecular markers or gene specific markers to overcome the Fusarium related biotic stresses.
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