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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 7, Issue:2, February, 2018

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2018.7(2): 2767-2770
DOI: https://doi.org/10.20546/ijcmas.2018.702.337


Breed Related Hospital Prevalence of Theileria annulata infection in Cattle-calves Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan, India
Pavan Goyal* and Anju Chahar
Department of Epidemiology and Preventive Veterinary Medicine, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan, India
*Corresponding author
Abstract:

One hundred cattle-calves, out of which 37 cross bred and 63 indigenous, were screened for Theileria annulata infection during October-2015 to September-2016 brought to Teaching Veterinary Clinical Complex, College of Veterinary and Animal Science, Bikaner. Blood samples were collected in ethylene diamine tetra acetic acid (EDTA @1mg/ml) vacutainers from all the suspected cattle-calves for genomic DNA isolation and subjected for confirmatory diagnosis by nested polymerase chain reaction (nPCR). By nPCR, 19 cross bred and 22 indigenous cattle-calves were found positive for Theileria annulata infection. Thus, the prevalence of Theileria annulata infection in cross bred and indigenous cattle-calves was 51.35% (19/37) and 34.92% (22/63), respectively.


Keywords: Theileria annulata, Cattle-calves, DNA, nPCR, Cross bred, Prevalence

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How to cite this article:

Pavan Goyal and Anju Chahar. 2018. Breed Related Hospital Prevalence of Theileria annulata infection in Cattle-calves Confirmed by Nested Polymerase Chain Reaction in Bikaner District of Rajasthan, India.Int.J.Curr.Microbiol.App.Sci. 7(2): 2767-2770. doi: https://doi.org/10.20546/ijcmas.2018.702.337
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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