National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The establishment of safe and effective fertility inhibitors for dogs must also be matched by evidence that non-surgical fertility control is an efficient, humane, and socially acceptable alternative to surgical sterilization for managing over abundant populations of dogs and their impact on human interests. The present study was undertaken to study the contraceptive effects of coumestrol in adult male dogs. Experimental group I was given 300 µg coumestrol dissolved in di-methyl sulfoxide orally in the commercial dog treats, on days 0, 7, 14, 21 and 28. Control group II was given DMSO alone as per above schedule. One week after the end of treatment period, dogs were anaesthetized with xylazine (2.0mg/kg B. weight) and ketamine HCl (10mg/kg B. weight). Testicles were removed and efferent ductules were collected and fixed in Bouin’s fixatives. Tissues were dehydrated in methanol and cleared in xylene, and then paraffin blocks were prepared. Sections were cut at 5-7 µ and stained with Harris-hematoxylin and Eosin. Histological examination of the testicular tissues revealed normal germinal epithelium. Therefore, it was concluded that oral feeding of 300 µg coumestrol did not have any adverse effect on male dog fertility.
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