National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The effects of different culture media and temperature on mycelial growth and sclerotial formation of Macrophomina phaseolina (Tassi) Goid inciting agent of castor root rot were studied in vitro. Eight different solid and liquid media were tested for their effect on mycelial growth and sclerotial formation of test fungus. Among the solid media, potato dextrose agar (90.00 mm) and Richard's agar (87.71 cm) were the best for fungus growth as well as for sclerotial formation followed by Elliott's medium (80.08 cm). Whereas in liquid media, richard's agar (862.00 mg) found excellent followed by potato dextrose agar (824.33 mg) for best mycelial growth and sclerotial formation of the test fungus. Different temperatures were tested for suitable fungal growth and it was observed that temperature range of 25 to 35 °C found optimum for growth and sclerotial formation. Whereas 30 °C (79.44 mm) was the ideal for the growth of the fungus. Identified suitable media could be used for growth and sclerotial formation study and temperature ranged could be used for epidemiological study.
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