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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 5, Issue:7, July, 2016
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
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Int.J.Curr.Microbiol.App.Sci.2016.5(7): 164-170
DOI: http://dx.doi.org/10.20546/ijcmas.2016.507.016


Purification and Characterization of a Novel CGTase from Alkaliphilic Bacillus flexus Isolated from Lonar lake, India
A.Shinde Vinod* and S.M. More
 Department of Microbiology, Yeshwant Mahavidyalaya, Nanded- 431602 (M. S.), India
*Corresponding author
Abstract:

Production and purification of Cyclodextrin glycosyltransferase (CGTase) from Alkaliphilic Bacillus flexus isolated from Lonar lake, India was investigated in present study. Production was carried out using medium containing starch, yeast extract, peptone MgSO4.7H2O. The crude enzyme was collected by centrifugation and partially purified using ammonium sulphate precipitation method. This partially purified enzyme was further purified using phenyl sepharose column chromatography .The enzyme obtained had molecular weight of 77.58 kDa which is confirmed by SDS PAGE and Mass spectroscopy.


Keywords: Cyclodextrin glycosyltransferase (CGTase); purification,Phenyl sepharose

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How to cite this article:

Shinde Vinod, A., and More, S.M. 2016. Purification and Characterization of a Novel CGTase from Alkaliphilic Bacillus flexus Isolated from Lonar lake, India.Int.J.Curr.Microbiol.App.Sci. 5(7): 164-170. doi: http://dx.doi.org/10.20546/ijcmas.2016.507.016
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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