National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The antagonistic activities of fluorescent Pseudomonas sp. isolated from rhizospheric soil of apple orchard of Himachal Pradesh were studied against two fungal pathogens viz., Dematophora necatrix and Phytophthora cactorum which were previously isolated from apple rhizosphere. The optimum conditions for growth and maximum production of antifungal activity by two selected fluorescent Pseudomonas sp. viz., An-2-nali and Pn-2-kho were detected. The influence of culture media, incubation time, incubation temperature and pH on growth and production of antifungal activity was investigated. From the results it is concluded that out of five media i.e. Potato dextrose agar (PDA), Kings’ B, Pigment production media (PPM), Yeast malt (YM) and 1/5 M 523, the maximum production of antifungal activity in terms of percent inhibition was observed in Yeast malt media after 96 hr of incubation. An-2-nali showed 37.93 %I against Dematophora necatrix and 38.46 %I against Phytophthora cactorum whereas isolate Pn-2-kho showed 43.10 %I against Dematophora necatrix and 36.53 %I against Phytophthora cactorum. The optimum conditions for production of antifungal activity by both the fluorescent Pseudomonas isolates were observed to be at temperature 28ËšC, pH 7 and an incubation time of 96 hr.
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