National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Bacteriological confirmation plays a key role in the diagnosis of tuberculosis. Many systems have been developed in the recent years apart from traditional microscopy and culture on Lowenstein Jensen (LJ) medium. The objective of this study is to compare the rate of detection of Mycobacteria by acid fast staining, LJ medium and Mycobacteria Growth Indicator Tube (MGIT). Hundred patients with clinical suspicion of pulmonary tuberculosis attending a tertiary care hospital over a period of one and a half years were enrolled in the study. Sputum samples were collected and acid fast smears were prepared. The specimens were decontaminated and cultured on LJ medium and MGIT. Growth obtained was recorded. Out of 100 suspected cases, acid fast bacilli were seen in 21 sputum samples by Ziehl-Neelsen (ZN) staining. In LJ medium culture 21 samples yielded growth and MGIT was positive in 23 samples. The sensitivity and specificity of MGIT in comparison with LJ medium culture were 100% and 97.5% respectively. MGIT culture showed faster rates of isolation compared to LJ medium with a mean detection time of 10.91days. Among the tuberculosis positive cases, 18 (78.2%) were males and 5 (21.7%) were females, with peak incidence (43.47%) in age group of 31-45years. Compared to conventional methods, MGIT showed a good sensitivity and specificity and faster rates of isolation. It can be used in the diagnosis of tuberculosis as a rapid and reliable technique.
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