National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
To observe the prevalence rate and pattern of drug sensitivity of bacterial infection among urinary isolates from rural part of West Bengal, India. A total of 9,763 urine samples of clinically suspected UTI were collected. The samples were inoculated on Blood agar, Mac Conkey's agar, and cystine lactose electrolyte deficient (CLED) agar and incubated at 37 °C for 24 hr and extended up to 48 hr in cases of no growth of any organism. Antibiotic susceptibility testing against the isolated aerobic bacteria was performed by Kirby Bauer’s disc diffusion method. Altogether 9,763 urine samples from tribal areas were collected for this study from January, 2013 to December, 2015. Total 2,710 (27.75%) samples were shown to be positive for growth of any bacteria. The dominant aerobic microorganisms isolated as the causative agents were E. coli (64.65%), Klebsiella spp. (16.27%), Pseudomonas aeruginosa (4.09%), Proteus spp. (3.21%), Staphylococcus aureus including coagulage negative Staphylococci (6.46%), Enterococcus spp. (1.00%) and other gram negative bacteria (4.32%). E. coli was the most common gram negative aerobic pathogen of UTI isolated in tribal population. In-vitro antibiotic susceptibility testing showed that the gram negative bacteria were sensitive to aminoglycosides (amikacin) and carbapenems (meropenem), while the gram positive isolates were sensitive to norfloxacin, gatifloxacin and levofloxacin. Isolation of the aerobic microorganisms from UTI patients of tribal population in rural part of West Bengal revealed E. coli as the most common bacterium in significant bacteriuria and amikacin is the most effective drug against the clinically isolated pathogens.
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