National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692 Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The study was carried out to assess the thermo stability of Newcastle Disease viruses (local isolates) which were isolated, characterised and provided by the Department of Veterinary Epidemiology and Preventive Medicine, West Bengal university of animal and Fishery Sciences. The thermo stability procedure was carried out at 250C for 36 days, 370C for 29 days, 400C for 30 days and 430C for 30 days respectively for each isolates. The results showed that all the five isolates tolerated 250C and 370C temperature for 36 days and 30 days respectively. The L4 isolate tolerated the 400C temperature for 18 days but the L5 isolate tolerated the 430C temperature for 12 days (showed HA titre 24 and EID50 107.5) was detected in 30 days heat exposure. The L5 isolate showed the longest viability after exposure to the highest temperature and can be used as seed virus for preparation of vaccine at room temperature after attenuation.
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