National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Cotyledonary explants of tomato cv. PKM-1 were co-cultivated with Agrobacterium tumefaciens strain, LBA4404 harbouring a codon-optimised chimeric cry2AX1 gene driven by enhanced CaMV35S promoter in pCAMBIA2300 vector backbone. Ninety six putative transgenic plants were regenerated, and the presence of the cry2AX1 gene in fifty eight plants was demonstrated by PCR analysis. ELISA showed that nine out of the fifty eight plants had detectable level of Cry2AX1 protein expression, which ranged from 0.030 to 0.388 µg/g of fresh tissue. Insect bioassay of transgenic T0 tomato plants using H. armigera neonates recorded a mortality of 16.67 to 100 per cent and showed significant reduction in leaf feeding and inhibition of growth in surviving larvae. The results demonstrated the potential of the chimeric cry2AX1 gene in developing H. armigera resistant transgenic tomato varieties.
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