Drug resistant Acinetobacter species increases the morbidity and mortality of hospitalized patients. This study was done with the aim of analysis of resistance pattern of Acinetobacter species in blood and respiratory tract sample of infected hospitalized patients. This study is a retrospective study of a tertiary care hospital and includes database of 54 patients with Acinetobacter species infection in blood and respiratory tract samples, isolated in six month period. An antibiogram was made for these patients along with their clinical details. Odd's ratio was calculated for each clinical condition of the patient. Rate of isolation of Acinetobacter species was 3.2% during six month period. 96.3% Acinetobacter species isolated were from ICU and 3.7% from ward patients 16.67% were MDR,72.22% were XDR, 5, 56% were PDR while only 5.56% were sensitive strains. 42.5% patients acquiring Acinetobacter infection had undergone some surgical procedure. Co-morbid conditions were present in 40.7% patients. An increased risk for resistance development was found only for hypertension. The mortality rate of the patients infected with Acinetobacter species was 55.55%. Interpretation: Drug resistant Acinetobacter species infection is taking foothold in intensive care settings because of pre-disposing conditions. A rigid adherence to antibiotic policies and infection control practices is essential to conquer the drug resistant Acinetobacter species.

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