National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Salvadora oleoides Decne belonging to family Salvadoraceae was selected for present study. This species is declining very rapidly due to various man made adverse activities like indiscriminate collection, over exploitation and loss of habitat. Therefore, in order to conserve and propagate this species, all modern micropropagation strategies were employed for rapid multiplication of this species. In this reports biotechnological technique especially micropropagation showed significant results from propagation point of view. Various explants like shoot tips, Nodal segments and cotyledonary nodes were cultured on Murashige and Skoog’s medium. There was a diverse response of different explants regarding concentration of different growth regulators. However, growth regulators used in combination supported better results in terms of per cent regeneration and number of shoots. The different explants obtained from in vitro grown seedlings, gave better results under higher concentration of growth regulators whereas explants obtained from field grown mature tree showed better results supplemented with lower concentration of growth regulators. Furthermore, explants obtained from in vitro grown seedlings bore out better in terms of per cent regeneration of shoots and number of shoot regenerated. Rhizogenesis was achieved on full strength MS medium fortified with auxins. The present research strongly recommends the maximum use of micropropagation techniques for rapid multiplication of true to type plants of Salvadora oleoides Decne.
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