Infectious bursal disease (IBD) or Gumboro causes heavy economic losses in poultry industry. Farmers are facing the problems of Gumboro even in the vaccinated flocks. The control of IBD depends on appropriate immunization schedule and maintenance of good hygienic conditions on the farm. For estimating optimum day of vaccination in broiler chickens serum samples were collected from 18 hatcheries situated in different parts belonging to Jind, Panipat and Hisar district of Haryana. Maternal antibody (MAb) titre of all the serum samples collected from different hatcheries along with day of vaccination was calculated as per Deventer formula. Statistics was applied on this raw data and the uniformity of day-old broiler chicks was expressed as percentage of coefficient of variation (CV %) which was in the range of 28.2% to 75.3%. The vast range of MAb titre (2 to 13128) in different serum samples gave wide range of day of vaccination as calculated for individual samples of all the hatcheries and it came to be in the range of 7 to 19 days of age of vaccination. According to Deventer formula a flock showing variation of > 4 days in the age of vaccination range needs to be vaccinated twice. Due to wide range of MAb titre, twice vaccination is advisable and the age of first vaccination came to be 9th day and age for booster vaccination came to be 16th day. However, further studies are required to strengthen the observations.

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