The bile is a lipid-rich sterile solution produced in the liver. Infection of biliary tract may occur in many conditions due to breakdown of human defence mechanisms and it is associated with post-operative complication that may prolong hospital stay hence present study was undertaken to detect the bacterial pathogens from bile and their antimicrobial sensitivity pattern. To study antimicrobial susceptibility pattern of organisms isolated from bile specimen and to know common pathogens isolated from bile during study period. To establish empirical therapy for biliary tract infection in our hospital, all the bile specimen received in Microbiology Department for culture and sensitivity were included in study. These specimens were processed as per standard protocol. Relevant patient data was collected. Prospective observational study six months, in this study period total 57 samples were studied Out of which 7 were sterile, in 34 samples single bacterial pathogen was grown and in 16 samples more than one bacterial pathogen was grown. Most common organisms isolated were Escherichia coli and Pseudomonas aeruginosa.

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