The present investigation was intended to optimize the solid substrate for the production of biomass of lactic acid bacteria using various dhals such as raw bengal gram dhal, roasted bengal gram dhal, black gram dhal, green gram dhal, hyacinth dhal (Avaraebele), masoor dhal, red gram dhal, soya bean dhals for growing Lactic Acid Bacteria using the solid state fermentation technique after screening for bacterial spores. The screened black gram dhal was selected which found to have lowest spore count of 1.52 log10 cfu/g then were exposed to hot air oven at 1000C for 1 h and sterilization at 1210C for 30 min for complete destruction of spores. The black gram dhal was added with various supplements each of skim milk powder, ash guard juice, carrot juice and tomato juice as sources of growth factors at the rate of 0.5, 1, 1.5 and 2%. The cultures selected for biomass production were dhai culture, yoghurt culture and acidophilus cultures added at 1% level of inoculum. The optimum growth was obtained when supplements were added each at 1% concentration with maximum viable counts for dahi culture was 8.41 log10 cfu/g while yoghurt culture of 8.98 log10 cfu/g and acidophilus culture of 9.47 log10 cfu/g.

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