Root architecture is critical for efficient nutrient acquisition. This necessitates the investigations on plant root system to identify genotype with high nitrogen uptake efficiency under target environment. A hydroponic culture was employed to standardize the media to be used for screening of wheat genotypes for Nitrogen Use Efficiency (NUE) using proven low nitrogen susceptible and tolerant genotypes. Four different modified Hoagland solutions (NH4NO3 media, NO3 complete, NO3 half, and N minus media) were used and five wheat genotypes namely, UASBW 11328, GW 322 and PDW 291, NARBADA 4 and C 306 were grown in hydroponic culture for 30 days. Results regarding analysis of variance for interactions of genotypes and nitrogen levels revealed that all the genotypes exhibited the significant difference among themselves for all the four different solutions. Over the different media, low N susceptible genotype C 306 exhibited significant difference for most of the traits. While over the genotypes, both half NO3 and N-minus recorded significant difference for root and shoot traits. Thus it was concluded that, half NO3 medium is the best to screen the wheat genotypes for screening for NUE under 50 per cent supply. Further, this method gives the way for quick initial screening of wheat genotypes for NUE.

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