Freshly harvested high moisture undried rice seeds will induce the deterioration rapidly, it affect the storability of the seed material. The present study was carried out to access the biochemical changes of high moisture and dried seeds taking place under cold and ambient storage condition. The freshly harvested high moisture rice seeds was harvested and stored under cold and ambient storage condition for 15 days in Department of Seed Science and Technology, Tamil Nadu Agricultural University, Coimbatore. In order to know the effectiveness of the storage condition the seeds drawn at 5, 10 and 15 days after storage and it was dried to optimum moisture content, followed by the enzymatic changes was carried out viz., dehydrogenase, α- amylase, catalase and peroxidase. The seeds under cold storage performed better than ambient storage condition. The enzymes which will helps the storability of seed was maximum in undried and dried seeds stored under cold storage condition viz., Dehydrogenase (0.174 and 0.773 OD value), α- amylase (1.38 and 1.43 mg maltose min.-1), due to lower respiratory activity in seeds, which was higher than the ambient storage condition. The rising of enzyme activity in undried and dried seeds stored under ambient condition such as Catalase (4.228 and 4.210 µg H2O2 mg-1 min-1), Peroxidase activity (2.27 and 2.12 m moltetraguaicol g-1), will shown signaling of seed deterioration.

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