The main source of microbial contamination of coastal waters is domestic waste water and sewage discharge. In this present investigation sea foods were collected from Ennore creek Chennai Coast. The sea foods like Catla, Malabar trevally, prawn, Threadfin bream, mussels and oysters were degutted and bacteria were isolated using selective and non-selective agar medium and further confirmed by biochemical tests. The organisms were identified to generic or group level according to Bergey's manual of systematic Bacteriology. Different group of fecal coliforms and Vibrio spp. have been isolated. Escherichia coli, Vibrio parahaemolyticus, Vibrio cholera, Staphylococcus aureus, Shigella dysentriae were the dominant species. Shell fishes ingest bacteria along with their feed which is dangerous to humans who eat the infected seafood. Sea food has to be handled, stored and processed properly.

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