Pseudomonas aeruginosa is responsible for the devastating nosocomial infections. Class C of cephalosporinase (AmpC β-lactamases) is important cause of multiple β-lactam resistance in P. aeruginosa. The aim of this study is to detect the AmpC β-lactamase production and Antimicrobial susceptibility pattern in Pseudomonas aeruginosa isolated from clinical samples in Tertiary health care Hospital. This study was conducted over a period of January (2016) to July (2016) in department of Microbiology, Sarojini Naidu Medical College, Agra (Uttar Pradesh), India. The organisms were identified on the basis of their culture characteristics and battery of Biochemical tests. Antimicrobial susceptibility of all the isolates was performed by the Kirby- Bauer disc-diffusion method. AmpC β-lactamase production was determined by the modified 3D test. 159 isolates of P. aeruginosa were obtained from different specimens. Maximum numbers of P. aeruginosa isolates were resistant to Piperacillin followed by Gentamycin, Cefepime and Ceftazidime. While most isolates were sensitive to Polymyxin-B, Colistin, Amikacin, Imipenem, and Meropenem. A high prevalence of AmpC β-lactamases production was observed amongst the isolates of P. aeruginosa as compared to other studies. Isolates of AmpC β-lactamases producing Pseudomonas aeruginosa have been continuously reported globally with some disparity in the rates of resistance.

Download this article as